A COMPARATIVE-STUDY OF TK6 HUMAN LYMPHOBLASTOID AND L5178Y MOUSE LYMPHOMA CELL-LINES IN THE IN-VITRO MICRONUCLEUS TEST

被引：42

作者：

ZHANG, LS ^{[1
]}

HONMA, M ^{[1
]}

HAYASHI, M ^{[1
]}

SUZUKI, T ^{[1
]}

MATSUOKA, A ^{[1
]}

SOFUNI, T ^{[1
]}

机构：

[1] NATL INST HYG SCI,DIV GENET & MUTAGENESIS,SETAGAYA KU,TOKYO 158,JAPAN

来源：

MUTATION RESEARCH LETTERS | 1995年 / 347卷 / 3-4期

关键词：

IN VITRO MICRONUCLEUS TEST; HUMAN LYMPHOBLASTOID CELLS; TK6; MOUSE LYMPHOMA CELLS; L5178Y;

D O I：

10.1016/0165-7992(95)00027-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Micronucleus induction was compared in human lymphoblastoid TK6 and mouse lymphoma L5178Y cell lines treated with model clastogens and spindle poisons, i.e., X-rays, methyl methanesulfonate, ethyl methanesulfonate, mitomycin C, colcemid, and vincristine. The spontaneous micronucleated cell (MNC) frequency was stable and reproducible in both cell lines. All clastogens and spindle poisons studied here induced micronuclei in both cell lines. They increased MNC frequency at lower concentrations or caused a greater increase at the same concentration in TK6 cells. These clastogens and spindle poisons, however, were also more toxic to TK6 than to L5178Y cells and when comparison was based on cytotoxicity, they showed more efficient MNC induction in L5178Y cells. In conclusion, neither cell line was superior to the other, and both of them can be used as target cells in the in vitro micronucleus assay.

引用

页码：105 / 115

页数：11

共 26 条

[1] MAMMALIAN-CELL GENE MUTATION ASSAYS WORKING GROUP-REPORT [J].

AARON, CS ;

BOLCSFOLDI, G ;

GLATT, HR ;

MOORE, M ;

NISHI, Y ;

STANKOWSKI, L ;

THEISS, J ;

THOMPSON, E .

MUTATION RESEARCH, 1994, 312 (03) :235-239

[2] CYTOKINESIS-BLOCK MICRONUCLEUS ASSAY WITH KINETOCHORE DETECTION IN COLCHICINE-TREATED HUMAN FIBROBLASTS [J].

ANTOCCIA, A ;

TANZARELLA, C ;

MODESTI, D ;

DEGRASSI, F .

MUTATION RESEARCH, 1993, 287 (01) :93-99

[3] MICRONUCLEI AND 3AB INDEX IN X-IRRADIATED HUMAN-LYMPHOCYTES IN G0 AND G1 PHASES [J].

CATENA, C ;

VILLANI, P ;

CONTI, D ;

RIGHI, E .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1994, 311 (02) :231-237

[4] MICRONUCLEI AND 3AB INDEX IN HUMAN AND CANINE LYMPHOCYTES AFTER IN-VITRO X-IRRADIATION [J].

CATENA, C ;

CONTI, D ;

VILLANI, P ;

NASTASI, R ;

ARCHILEI, R ;

RIGHI, E .

MUTATION RESEARCH, 1994, 312 (01) :1-8

[5] HIGH-CAPACITY INVITRO MICRONUCLEUS ASSAY FOR ASSESSMENT OF CHROMOSOME-DAMAGE - RESULTS WITH QUINOLONE NAPHTHYRIDONE ANTIBACTERIALS [J].

CIARAVINO, V ;

SUTO, MJ ;

THEISS, JC .

MUTATION RESEARCH, 1993, 298 (04) :227-236

[6] A COMPARISON OF THE AGAR CLONING AND MICROTITRATION TECHNIQUES FOR ASSAYING CELL-SURVIVAL AND MUTATION FREQUENCY IN L5178Y MOUSE LYMPHOMA-CELLS [J].

COLE, J ;

ARLETT, CF ;

GREEN, MHL ;

LOWE, J ;

MURIEL, W .

MUTATION RESEARCH, 1983, 111 (03) :371-386

[7] MICRONUCLEUS, CHROMOSOME ABERRATION, AND SMALL-COLONY TK MUTANT ANALYSIS TO QUANTITATE CHROMOSOMAL DAMAGE IN L5178Y MOUSE LYMPHOMA-CELLS [J].

DOERR, CL ;

HARRINGTONBROCK, K ;

MOORE, MM .

MUTATION RESEARCH, 1989, 222 (03) :191-203

[8] MEASUREMENT OF MICRONUCLEI IN LYMPHOCYTES [J].

FENECH, M ;

MORLEY, AA .

MUTATION RESEARCH, 1985, 147 (1-2) :29-36

[9] CHROMOSOME-ABERRATIONS INDUCED IN-VITRO - MECHANISMS, DELAYED EXPRESSION, AND INTRIGUING QUESTIONS [J].

GALLOWAY, SM .

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 1994, 23 :44-53

[10] COMPARISON OF COMMON GENE MUTATION TESTS IN MAMMALIAN-CELLS IN CULTURE - A POSITION PAPER OF THE GUM-COMMISSION-FOR-THE-DEVELOPMENT-OF-GUIDELINES-FOR-GENOTOXICITY-TESTING [J].

GLATT, H .

MUTATION RESEARCH-ENVIRONMENTAL MUTAGENESIS AND RELATED SUBJECTS, 1994, 313 (01) :7-20

← 1 2 3 →