CAPILLARY ISOTACHOPHORESIS WITH UV AND TANDEM MASS-SPECTROMETRIC DETECTION FOR PEPTIDES AND PROTEINS

被引：50

作者：

SMITH, RD

FIELDS, SM

LOO, JA

BARINAGA, CJ

UDSETH, HR

EDMONTS, CG

机构：

[1] Chemical Methods and Separations Group, Chemical Sciences Department, Pacific Northwest Laboratory, Richland, Washington

[2] Dow Chemical, Pittsburgh, California, 94656

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 09期

关键词：

D O I：

10.1002/elps.1150110907

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of capillary isotachophoresis (CITP) and combined CITP‐mass spectrometry (MS) for peptides and proteins is demonstrated. Separation of simple peptide mixtures, as well as enzymatic digets of proteins, is also reported using CITP with UV detection. The potential utility of CITP for proteins is demonstrated. Initial studies of combined CITP‐MS of enzymatic digests is also demonstrated, showing the potential for rapid sequence determination. Copyright © 1990 VCH Verlagsgesellschaft mbH

引用

页码：709 / 717

页数：9

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