A NEW THERMOSTABLE ENDOGLUCANASE, ACIDOTHERMUS-CELLULOLYTICUS E1 - SYNERGISM WITH TRICHODERMA-REESEI CBH-I AND COMPARISON TO THERMOMONOSPORA-FUSCA E(5)

被引：46

作者：

BAKER, JO ^{[1
]}

ADNEY, WS ^{[1
]}

NIEVES, RA ^{[1
]}

THOMAS, SR ^{[1
]}

WILSON, DB ^{[1
]}

HIMMEL, ME ^{[1
]}

机构：

[1] CORNELL UNIV, BIOCHEM MOLEC & CELL BIOL SECT, ITHACA, NY USA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1994年 / 45-6卷

关键词：

ACIDOTHERMUS CELLULOLYTICUS E1; THERMOMONOSPORA FUSCA E(5); TRICHODERMA REESEI CBH I; SYNERGISM; ENDOGLUCANASE;

D O I：

10.1007/BF02941803

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new thermostable endoglucanase, Acidothermus cellulolyticus Fl, and another bacterial endoglucanase, E, from Thermomonospora fusca, each exhibit striking synergism with a fungal cellobiohydrolase (Trichoderma reesei CBH I) in the saccharification of microcrystalline cellulose. In neither case did the ratio of endoglucanase to exoglucanase that demonstrated maximum synergism coincide exactly with the ratio that actually released the maximum quantity of soluble sugar for a given total cellulase loading. The difference between the two ratios, after significant hydrolysis of the substrate, was considerably larger in the case of A. cellulolyticus E1. For both endoglucanase pairings with CBH I, the offset between the ratio for maximum synergism and the ratio for maximal soluble sugar production was found to be a function of digestion time.

引用

页码：245 / 256

页数：12

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