TRANSFER OF HYGROMYCIN RESISTANCE INTO BRASSICA-NAPUS USING TOTAL DNA OF A TRANSGENIC BRASSICA-NIGRA LINE

被引：12

作者：

GOLZ, C

KOHLER, F

SCHIEDER, O

机构：

[1] Institut für Angewandte Genetik, FU Berlin, Berlin 33, 1000

来源：

PLANT MOLECULAR BIOLOGY | 1990年 / 15卷 / 03期

关键词：

B; nigra; Brassica napus; direct gene transfer; plasmid DNA; regeneration; total DNA;

D O I：

10.1007/BF00019164

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The successful transfer of a marker gene (hpt gene) from Brassica nigra into B. napus via direct gene transfer was demonstrated. Total DNA was isolated from a hygromycin-resistant callus line, which contained three to five copies of the hpt gene. This line had been produced via direct gene transfer with the hygromycin resistance-conferring plasmid pGL2. The treatment of B. napus protoplasts with genomic DNA of B. nigra (HygR) resulted in relative transformation frequencies of 0.1-0.4%. Similar transformation rates were obtained in direct gene transfer experiments using B. napus protoplasts and plasmid pGL2. © 1990 Kluwer Academic Publishers.

引用

页码：475 / 483

页数：9

共 24 条

[1] PROTOPLAST CULTURE AND PLANT-REGENERATION FROM BRASSICA-CARINATA BRAUN [J].