IN-VITRO FERTILIZATION OF OOCYTES BY 37-YEAR-OLD CRYOPRESERVED BOVINE SPERMATOZOA

被引：34

作者：

LEIBO, SP ^{[1
]}

SEMPLE, ME ^{[1
]}

KROETSCH, TG ^{[1
]}

机构：

[1] UNITED BREEDERS INC,GUELPH,ON,CANADA

来源：

THERIOGENOLOGY | 1994年 / 42卷 / 08期

关键词：

BOVINE SPERMATOZOA; CRYOPRESERVATION; LONG-TERM STORAGE;

D O I：

10.1016/0093-691X(94)90245-E

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bull spermatozoa that had been frozen in 1957 was tested for fertility after 37 years in storage. Two specimens of this ''vintage'' spermatozoa exhibited normal motility when thawed and successfully fertilized bovine oocytes in vitro. Of 670 oocytes incubated with vintage spermatozoa, 149 (22%) cleaved into 2-cell embryos, of which 32 (5% of the total number) developed into normal blastocysts.

引用

页码：1257 / 1262

页数：6

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