USE OF LACTATE-DEHYDROGENASE TO EVALUATE THE ANTIVIRAL ACTIVITY AGAINST INFLUENZA-A VIRUS

被引：34

作者：

WATANABE, W ^{[1
]}

SUDO, K ^{[1
]}

ASAWA, S ^{[1
]}

KONNO, K ^{[1
]}

YOKOTA, T ^{[1
]}

SHIGETA, S ^{[1
]}

机构：

[1] FUKUSHIMA MED COLL,DEPT MICROBIOL,FUKUSHIMA 96012,JAPAN

来源：

JOURNAL OF VIROLOGICAL METHODS | 1995年 / 51卷 / 2-3期

关键词：

LACTATE DEHYDROGENASE (LDH); MTT METHOD;

D O I：

10.1016/0166-0934(94)00103-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The detection of lactate dehydrogenase (LDH) can be used to evaluate efficiently anti-influenza A virus agents. LDH levels in the virus-infected Madin-Darby canine kidney cell cultures were significantly higher than in controls, were in proportion to the degree of virus infection, and corresponded to a decrease in mitochondrial dehydrogenase activity as assayed using a tetrazolium colorimetric assay (MTT method). The EC(50) value and cytotoxicity of ribavirin, 3-deazaguanine, pyrazofurin, and carbodine against influenza A virus as measured by the LDH detection method was equivalent to that derived by the MTT method.

引用

页码：185 / 191

页数：7

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