MEASUREMENT OF ADHESION AND SPREADING KINETICS OF BABY HAMSTER-KIDNEY AND HYBRIDOMA CELLS USING AN INTEGRATED OPTICAL METHOD

被引：30

作者：

LI, SY ^{[1
]}

RAMSDEN, JJ ^{[1
]}

PRENOSIL, JE ^{[1
]}

HEINZLE, E ^{[1
]}

机构：

[1] ETH ZURICH, DEPT CHEM ENGN, CH-8092 ZURICH, SWITZERLAND

来源：

BIOTECHNOLOGY PROGRESS | 1994年 / 10卷 / 05期

关键词：

D O I：

10.1021/bp00029a010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

A novel optical method was used for quantitative characterization and continuous measurement of both the adhesion and spreading of mammalian cells on inorganic surfaces. It is based upon the effective refractive index change caused by cells when they adhere to a planar optical waveguide. We have applied this technique to measure the kinetics of the adhesion and spreading processes of baby hamster kidney (BHK) cells adhering to surfaces coated with fibronectin and under different culture conditions (PBS, medium, serum, EDTA). In comparison, hybridoma cells are only adsorbed to the surface and do not spread at all. Moreover, this technique also allows the mass of an adsorbed protein adlayer to be determined very precisely and thus provides a valuable tool for screening suitable substrata as well as determining the influence of different culture conditions on cell adhesion and spreading. This sensitive test for substrate influence could be important in toxicity tests using adherent animal cells.

引用

页码：520 / 524

页数：5

共 14 条

[1]

Bamgrover D, 1986, MAMMALIAN CELL TECHN, P131

[2]

Butler M., 1988, ANIMAL CELL BIOTECHN, V3, P284

[3]

ROLE OF CELL-SHAPE IN GROWTH-CONTROL [J].