RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS AMONG THE FLAGELLAR GENES OF THE LIOR HEAT-LABILE SEROGROUP REFERENCE STRAINS AND FIELD STRAINS OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI

被引：19

作者：

BURNENS, AP ^{[1
]}

WAGNER, J ^{[1
]}

LIOR, H ^{[1
]}

NICOLET, J ^{[1
]}

FREY, J ^{[1
]}

机构：

[1] LAB CTR DIS CONTROL,OTTAWA,ON K1A 0L2,CANADA

来源：

EPIDEMIOLOGY AND INFECTION | 1995年 / 114卷 / 03期

关键词：

D O I：

10.1017/S0950268800052134

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Several typing systems have been described for Campylobacter jejuni and C. coli, to assess the complex epidemiology of these important enteric pathogens. In the present study two typing methods, slide agglutination according to the Lior scheme, and the demonstration of restriction-fragment length polymorphisms (RFLP) of flagellar genes, have been used in parallel on a set of 194 strains. This set comprised 118 sere-reference strains of C. jejuni and C. coli of the Lior scheme, as well as 76 clinical isolates. All isolates were serotyped and subjected to PCR for amplification of flagellar genes, and the PCR product was restricted with Abu. I. Flagellar genes could be amplified in 152 strains. Among 85 seroreference strains, 74 different RFLP patterns were observed, and among 67 clinical isolates, there were 36 patterns. There was only limited correlation between flagellar RFLP and the Lior serogroup, and the variability of patterns in serogroups HL2 and HL4 were as marked. as the variability between serogroups. Flagellar gene RFLP patterns are shown to be stable, highly discriminatory epidemiologic markers.

引用

页码：423 / 431

页数：9

共 21 条

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