SIMULTANEOUS AND ITERATIVE WEIGHTED REGRESSION-ANALYSIS OF TOXICITY TESTS USING A MICROPLATE READER

被引：14

作者：

GALGANI, F

CADIOU, Y

GILBERT, F

机构：

[1] IFREMER, 44037 Nantes Cedex, Rue de l'Ile d'Yeu

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 1992年 / 23卷 / 02期

关键词：

D O I：

10.1016/0147-6513(92)90061-7

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A system is described for determination of LC50 or IC50 by an iterative process based on data obtained from a plate reader using a marine unicellular alga as a target species. The esterase activity of Tetraselmis suesica on fluorescein diacetate as a substrate was measured using a fluorescence titerplate. Simultaneous analysis of results was performed using an iterative process adopting the sigmoid function Y = y 1 (dose of toxicant/IC50)slope for dose-response relationships. IC50 (± SEM) was estimated (P < 0.05). An application with phosalone as a toxicant is presented. © 1992.

引用

页码：237 / 243

页数：7

共 12 条

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