METABOLISM OF TESTOSTERONE DURING INVITRO TRANSPORT ACROSS CACO-2 CELL MONOLAYERS - EVIDENCE FOR BETA-HYDROXYSTEROID DEHYDROGENASE-ACTIVITY IN DIFFERENTIATED CACO-2 CELLS

Testosterone has previously been used as a model compound for the determination of unstirred water layer thickness in the CACO-2 transport model. We have found, however, that testosterone is metabolized during in vitro transport across the CACO-2 cell monolayers. This suggests that testosterone is not an ideal model substance. Testosterone is metabolized to androstenedione, indicating the formation of 17-beta-hydroxysteroid dehydrogenase by differentiated CACO-2 cells. No reverse metabolism is observed, thus androstenedione is considered superior to testosterone for determination of unstirred water layer thickness in the CACO-2 system. Permeability coefficients for testosterone and androstenedione obtained under identical transport conditions were 66 (+/- 7) * 10(-6) (n = 26) and 84 (+/- 7) * 10(-6) (n = 9) cm/sec. respectively. The unstirred water layer thicknesses at different agitation rates are determined for the CACO-2 transport model used in our laboratory utilizing androstenedione as a model compound. The system is capable of controlling the water layer thickness from about 200 to 1000 mum.