ANALYTICAL AND MICROPREPARATIVE SEPARATION OF PEPTIDES BY CAPILLARY ZONE ELECTROPHORESIS USING DISCONTINUOUS BUFFER SYSTEMS

被引:119
作者
SCHWER, C
LOTTSPEICH, F
机构
[1] Department of Protein Chemistry, Max-Planck-Institute for Biochemistry D(W)
来源
JOURNAL OF CHROMATOGRAPHY | 1992年 / 623卷 / 02期
关键词
D O I
10.1016/0021-9673(92)80375-5
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The tiny injection volumes that arc usually necessary to maintain the high efficiency of capillary zone electrophoresis present a major problem if only limited sample amounts are available. To increase the sample load, discontinuous buffer systems were developed that allow the on-column concentration of dilute samples. Injection volumes can be increased in this way by at least a factor of 30. These stacking systems were applied to the analysis of tryptic peptides, to the purity checking of high-performance liquid chromatographic fractions and for the micropreparative separation of peptides with subsequent amino acid sequence analysis.
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页码:345 / 355
页数:11
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