STABILIZATION OF ERYTHROCYTES BY ALDEHYDES AND SUITABILITY OF CHICKEN IGY FOR THE DETECTION OF POTATO-VIRUS X(PVX) IN AVIDIN-BIOTIN ENHANCED REVERSE PASSIVE HEMAGGLUTINATION

被引：3

作者：

GROSSMANN, A ^{[1
]}

EGGERSSCHUMACHER, G ^{[1
]}

SANDER, E ^{[1
]}

机构：

[1] UNIV TUBINGEN,INST BIOL,D-72076 TUBINGEN,GERMANY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1995年 / 179卷 / 02期

关键词：

REVERSE PASSIVE HEMAGGLUTINATION; POTATO VIRUS X; AVIDIN-BIOTIN; FIXATION METHOD; IGY-TYPE ANTIBODY; CHICKEN; VIROLOGY;

D O I：

10.1016/0022-1759(94)00291-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Methods are described for the detection of potato virus X (PVX) by reverse passive haemagglutination (RPH) by means of polyclonal antiviral antibodies coupled to sheep red blood cells (sRBC) and the chromic chloride method. The cells were stabilized with pyruvic aldehyde, thus providing a stock suspension for numerous coupling experiments lasting several months. Anti-PVX IgY, which is readily isolated in large amounts from the egg yolk of immunized chickens, was used in an avidin-biotin enhanced RPH assay with stabilized sRBC. With this method the PVX detection rate achieved was comparable to that of RPH assays using fresh non-fixed sRBC. In addition, avidin-coated sRBC could be stored for weeks at 4 degrees C and subsequently used for coupling with biotinylated IgY.

引用

页码：243 / 250

页数：8

共 21 条

[1]

ASSAYS USING RED CELL-LABELED ANTIBODIES [J].