LACK OF MESSENGER-RNA AND DYSTROPHIN EXPRESSION IN DMD PATIENTS 3 MONTHS AFTER MYOBLAST TRANSFER

被引：49

作者：

MORANDI, L

BERNASCONI, P

GEBBIA, M

MORA, M

CROSTI, F

MANTEGAZZA, R

CORNELIO, F

机构：

[1] Department of Neuromuscular Diseases, Istituto Nazionale Neurologico C. Besta, 20133 Milan

来源：

NEUROMUSCULAR DISORDERS | 1995年 / 5卷 / 04期

关键词：

DMD; MYOBLAST TRANSFER; DYSTROPHIN MESSENGER-RNA;

D O I：

10.1016/0960-8966(94)00070-P

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

We report our experience on myoblast transplantation in three Duchenne muscular dystrophy patients. Pure myoblasts (55 x 10(6) per patient) from HLA-matched donors, were injected into a tibialis anterior and the controlateral muscle was sham injected. Three months after transplantation, biopsies from the injected muscles were negative for dystrophin expression by immunocytochemistry. Reverse transcriptase-PCR (RT-PCR) failed to amplify any fragments of the deleted regions. This result confirms that myoblast transplantation is feasible, although the efficacy of this therapeutic approach is poor.

引用

页码：291 / 295

页数：5

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