ESTRADIOL-17-BETA STIMULATES DNA-SYNTHESIS IN RAT GRANULOSA-CELLS - ACTION MEDIATED BY TRANSFORMING GROWTH-FACTOR-BETA

被引：53

作者：

BENDELL, JJ ^{[1
]}

DORRINGTON, J ^{[1
]}

机构：

[1] UNIV TORONTO,INST SCI & ENGN,TORONTO M5G 1L6,ONTARIO,CANADA

来源：

ENDOCRINOLOGY | 1991年 / 128卷 / 05期

关键词：

D O I：

10.1210/endo-128-5-2663

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Estradiol-17-beta is a mitogen in vivo for the proliferation of granulosa cells in the rat ovary. In this study we have shown that estradiol-17-beta acts on cultures of immature rat granulosa cells to stimulate DNA synthesis. DNA synthesis stimulated by estradiol-17-beta was enhanced by FSH. We have shown previously that transforming growth factor-beta (TGF-beta) stimulates DNA synthesis in rat granulosa cells in vitro and that this effect is augmented by FSH. The mitogenic action of estradiol-17-beta was mediated, at least in part, by a TGF-beta-like factor since in the presence of a neutralizing antibody to TGF-beta, DNA synthesis was attenuated. This conclusion was further supported by the novel finding that estradiol-17-beta stimulated the secretion of a TGF-beta-like factor by rat granulosa cells in culture. In summary, the evidence suggests that the actions of estradiol-17-beta and TGF-beta on DNA synthesis are interrelated, and that a TGF-beta-like factor mediates the mitogenic effects of estradiol-17-beta on rat granulosa cells.

引用

页码：2663 / 2665

页数：3

共 14 条

[1] EVIDENCE FOR A FSH DEPENDENT SECRETION OF A RECEPTOR REACTIVE TRANSFORMING GROWTH-FACTOR BETA-LIKE MATERIAL BY IMMATURE SERTOLI CELLS IN PRIMARY CULTURE [J].

BENAHMED, M ;

COCHET, C ;

KERAMIDAS, M ;

CHAUVIN, MA ;

MORERA, AM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 154 (03) :1222-1231

[2] RAT THECAL INTERSTITIAL-CELLS SECRETE A TRANSFORMING GROWTH FACTOR-BETA-LIKE FACTOR THAT PROMOTES GROWTH AND DIFFERENTIATION IN RAT GRANULOSA-CELLS [J].

BENDELL, JJ ;

DORRINGTON, J .

ENDOCRINOLOGY, 1988, 123 (02) :941-948

[3] HUMAN PLATELET-DERIVED GROWTH-FACTOR PREPARATIONS CONTAIN A SEPARATE ACTIVITY WHICH POTENTIATES FOLLICLE-STIMULATING HORMONE-MEDIATED INDUCTION OF LUTEINIZING-HORMONE RECEPTOR IN CULTURED RAT GRANULOSA-CELLS - EVIDENCE FOR TRANSFORMING GROWTH FACTOR-BETA [J].

BLAIR, EI ;

KIM, IC ;

ESTES, JE ;

KESKIOJA, J ;

SCHOMBERG, DW .

ENDOCRINOLOGY, 1988, 123 (04) :2003-2008

[4] TRANSFORMING GROWTH-FACTOR BETA-STIMULATING AND FOLLICLE-STIMULATING-HORMONE PROMOTE RAT GRANULOSA-CELL PROLIFERATION [J].

DORRINGTON, J ;

CHUMA, AV ;

BENDELL, JJ .

ENDOCRINOLOGY, 1988, 123 (01) :353-359

[5] ESTRADIOL-17-BETA BIOSYNTHESIS IN CULTURED GRANULOSA-CELLS FROM HYPOPHYSECTOMIZED IMMATURE RATS - STIMULATION BY FOLLICLE-STIMULATING HORMONE [J].

DORRINGTON, JH ;

MOON, YS ;

ARMSTRONG, DT .

ENDOCRINOLOGY, 1975, 97 (05) :1328-1331

[6]

ERICKSON GF, 1984, SEMINARS REPRODUCTIV, V2, P223

[7] ESTROGEN AND FOLLICLE STIMULATING HORMONE INTERACTIONS ON FOLLICLE GROWTH IN RATS [J].

GOLDENBERG, RL ;

ROSS, GT ;

VAITUKAITIS, JL .

ENDOCRINOLOGY, 1972, 90 (06) :1492-+

[8] EFFECTS OF EPIDERMAL AND FIBROBLAST GROWTH-FACTORS ON REPLICATIVE LIFESPAN OF CULTURED BOVINE GRANULOSA-CELLS [J].

GOSPODAROWICZ, D ;

BIALECKI, H .

ENDOCRINOLOGY, 1978, 103 (03) :854-865

[9] FIBROBLAST AND EPIDERMAL GROWTH-FACTORS ARE MITOGENIC AGENTS FOR CULTURED GRANULOSA-CELLS OF RODENT, PORCINE, AND HUMAN-ORIGIN [J].

GOSPODAROWICZ, D ;

BIALECKI, H .

ENDOCRINOLOGY, 1979, 104 (03) :757-764

[10] ESTROGEN BINDING, RECEPTOR MESSENGER-RNA, AND BIOLOGIC RESPONSE IN OSTEOBLAST-LIKE OSTEO-SARCOMA CELLS [J].

KOMM, BS ;

TERPENING, CM ;

BENZ, DJ ;

GRAEME, KA ;

GALLEGOS, A ;

KORC, M ;

GREENE, GL ;

OMALLEY, BW ;

HAUSSLER, MR .

SCIENCE, 1988, 241 (4861) :81-84

← 1 2 →