APPLICATION OF REVERSED-PHASE MEDIUM-PRESSURE LIQUID-CHROMATOGRAPHY TO THE ISOLATION, SEPARATION AND AMINO-ACID-ANALYSIS OF 2 CLOSELY RELATED PEPTIDE TOXINS OF THE CYANOBACTERIUM MICROCYSTIS-AERUGINOSA STRAIN PCC-7806

被引：9

作者：

CREMER, J

HENNING, K

机构：

[1] Institute of Microbiology, Federal Armed Forces Medical Academy, W-8000 Munich 45

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 587卷 / 01期

关键词：

D O I：

10.1016/0021-9673(91)85199-P

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A mixture of two compounds, related to cyanoginosin-LR, was isolated from the cyanobacterium Microcystis aeruginosa strain PCC 7806. Using an optimized reversed-phase fast protein liquid chromatography (RP-FPLC) method, up to 1.5 mg of this toxin mixture could be separated into two distinct compounds by a single preparative scale run. To perform an amino acid analysis of the two components, the hydrolysates derivatized with phenylisothiocyanate were analysed separately by RP-FPLC. A method was developed which could be used for the automatic sequential analysis of up to fourteen samples. The amino acid analysis by RP-FPLC was linear in the range 1-5 nmol. On the basis of this analysis, the two compounds differ by only a methylated versus a demethylated aspartate. Only six of the seven amino acids known for cyanoginosin-LR could be reliably detected.

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页码：71 / 80

页数：10

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