LIQUID-CHROMATOGRAPHIC MONITORING OF PSEUDOCHOLINESTERASE ACTIVITY - COMPARISON OF METHODS

被引:3
作者
CHELMINSKABERTILSSON, M [1 ]
ALLENMARK, S [1 ]
机构
[1] GOTHENBURG UNIV,MICROBIOL CHEM LAB,GULDHEDSGATAN 10A,S-41346 GOTHENBURG,SWEDEN
来源
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 575卷 / 02期
关键词
D O I
10.1016/0378-4347(92)80151-F
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In order to study the activity of pseudocholinesterase in vitro, two liquid chromatographic techniques have been developed. One is based on phase transfer-catalyzed (PTC) esterification of the carboxylic acid formed during hydrolysis of the substrate, and the other on the use of a radioisotopically labeled substrate. In both cases, the substrate used was a long-chain choline ester. The PTC method. utilizing (N-9-acridinyl)bromoacetamide as a fluorogenic labeling reagent in an aqueous-organic two-phase system, gives esters with very high fluorescence intensity. The radiochromatographic method makes use of on-line radioactivity monitoring of the substrate and product in order to follow the hydrolysis reaction. In both methods reversed-phase liquid chromatography is used. A method for the synthesis of H-3-labeled choline esters is also described. Both techniques are compared with regard to sensitivity, reproducibility and practical considerations.
引用
收藏
页码:237 / 242
页数:6
相关论文
共 20 条
[1]  
AGUSTINSSON KB, 1971, METHODS BIOCH ANAL A, P217
[2]   DETERMINATION OF CARBOXYLIC-ACIDS BY LIQUID-CHROMATOGRAPHY AFTER PHASE-TRANSFER-CATALYZED FLUOROGENIC LABELING [J].
ALLENMARK, S ;
CHELMINSKABERTILSSON, M .
JOURNAL OF CHROMATOGRAPHY, 1988, 456 (02) :410-416
[3]   N-(9-ACRIDINYL)-BROMOACETAMIDE - A POWERFUL REAGENT FOR PHASE-TRANSFER-CATALYZED FLUORESCENCE LABELING OF CARBOXYLIC-ACIDS FOR LIQUID-CHROMATOGRAPHY [J].
ALLENMARK, S ;
CHELMINSKABERTILSSON, M ;
THOMPSON, RA .
ANALYTICAL BIOCHEMISTRY, 1990, 185 (02) :279-285
[4]  
[Anonymous], [No title captured]
[5]  
BAKER SA, 1980, ANAL BIOCHEM, V107, P116
[6]  
DUNGES W, 1977, ANAL CHEM, V49, P442
[7]  
DUNGES W, 1977, FRESEN Z ANAL CHEM, V228, P361
[8]  
FARINOTTI R, 1983, J CHROMATOGR, V269, P81, DOI 10.1016/S0021-9673(01)90788-5
[9]  
GOMORI G, 1948, P SOC EXP BIOL MED, V68, P354
[10]  
HIRAI T, 1991, LIQUID CHROMATOGRAPH, P169