A RAPID METHOD FOR MEASURING ORGANELLE-SPECIFIC SUBSTRATE TRANSPORT IN HOMOGENATES FROM PLANT-TISSUES

被引：34

作者：

FLUGGE, UI

WEBER, A

机构：

来源：

PLANTA | 1994年 / 194卷 / 02期

关键词：

METABOLITE TRANSPORT; PLANT CELL MEMBRANES; RECONSTITUTION (MEMBRANES);

D O I：

10.1007/BF01101676

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A rapid method is described for measuring organelle-specific metabolite transport systems in crude homogenates from plants. The tissues were homogenized in liquid nitrogen, extracted with buffer and reconstituted into artificial membranes. The method allowed demonstration of the known different substrate specificities of chloroplast triose phosphate/phosphate translocators from C-3- and C-4-plants, of the triose phosphate/phosphate translocator from non-green tissue, and of the dicarboxylate translocator. It thus by-passes the necessity to isolate intact plant organelles and, in addition, only a low amount of tissue material is required for transport measurements.

引用

收藏

页码：181 / 185

页数：5

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