ART UPDATED 2-DIMENSIONAL GEL DATABASE OF RAT-LIVER PROTEINS USEFUL IN GENE-REGULATION AND DRUG EFFECT STUDIES

被引：48

作者：

ANDERSON, NL

ESQUERBLASCO, R

HOFMANN, JP

MEHEUS, L

RAYMACKERS, J

STEINER, S

WITZMANN, F

ANDERSON, NG

机构：

[1] INNOGENET NV,GHENT,BELGIUM

[2] SANDOZ PHARMA LTD,DRUG SAFETY ASSESSMENT,BASEL,SWITZERLAND

[3] INDIANA UNIV PURDUE UNIV,MOLEC ANAT LAB,COLUMBUS,IN

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 10期

关键词：

2-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS; LIVER; MAP; IDENTIFICATION; CALIBRATION;

D O I：

10.1002/elps.11501601324

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have improved upon the reference two-dimensional (2-D) electrophoretic map of rat liver proteins originally published in 1991 (N. L. Anderson et al., Electrophoresis 1991, 12, 907-930). A total of 53 proteins (102 spots) are now identified, many by microsequencing. In most cases, spots cut from wet, Coomassie Blue stained '2-D gels were submitted to internal tryptic digestion [2], and individual peptides, separated by high-performance liquid chromatography (HPLC), were sequenced using a Perkin-Elmer 477A sequenator. Additional spots were identified using specific antibodies.

引用

收藏

页码：1977 / 1981

页数：5

相关论文

共 10 条

[1] A 2-DIMENSIONAL GEL DATABASE OF RAT-LIVER PROTEINS USEFUL IN GENE-REGULATION AND DRUG EFFECTS STUDIES [J].

ANDERSON, NL ;

ESQUERBLASCO, R ;

HOFMANN, JP ;

ANDERSON, NG .

ELECTROPHORESIS, 1991, 12 (11) :907-930

[2] COVALENT PROTEIN MODIFICATIONS AND GENE-EXPRESSION CHANGES IN RODENT LIVER FOLLOWING ADMINISTRATION OF METHAPYRILENE - A STUDY USING 2-DIMENSIONAL ELECTROPHORESIS [J].

ANDERSON, NL ;

COPPLE, DC ;

BENDELE, RA ;

PROBST, GS ;

RICHARDSON, FC .

FUNDAMENTAL AND APPLIED TOXICOLOGY, 1992, 18 (04) :570-580

[3] THE HEPATOCARCINOGEN METHAPYRILENE BUT NOT THE ANALOG PYRILAMINE INDUCES SUSTAINED HEPATOCELLULAR REPLICATION AND PROTEIN ALTERATIONS IN F344 RATS IN A 13-WEEK FEED STUDY [J].

CUNNINGHAM, ML ;

PIPPIN, LL ;

ANDERSON, NL ;

WENK, ML .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 1995, 131 (02) :216-223

[4]

GASPARIC V, 1978, Patent No. 4130470

[5] ISOELECTRIC-FOCUSING IN IMMOBILIZED PH GRADIENTS IN PRESENCE OF UREA AND NEUTRAL DETERGENTS [J].

GIANAZZA, E ;

ARTONI, G ;

RIGHETTI, PG .

ELECTROPHORESIS, 1983, 4 (05) :321-326

[6] AN IMPROVED PROTOCOL FOR TWO-DIMENSIONAL MAPS OF SERUM-PROTEINS WITH IMMOBILIZED PH GRADIENTS IN THE 1ST DIMENSION [J].

GIANAZZA, E ;

ASTRUATESTORI, S ;

GIACON, P ;

RIGHETTI, PG .

ELECTROPHORESIS, 1985, 6 (07) :332-339

[7] IMPROVED HORIZONTAL TWO-DIMENSIONAL ELECTROPHORESIS WITH HYBRID ISOELECTRIC-FOCUSING IN IMMOBILIZED PH GRADIENTS IN THE 1ST-DIMENSION AND LAYING-ON TRANSFER TO THE 2ND-DIMENSION [J].

GORG, A ;

POSTEL, W ;

GUNTHER, S ;

WESER, J .

ELECTROPHORESIS, 1985, 6 (12) :599-604

[8] A COMPARATIVE-STUDY OF MOUSE-LIVER PROTEINS ARYLATED BY REACTIVE METABOLITES OF ACETAMINOPHEN AND ITS NONHEPATOTOXIC REGIOISOMER, 3'-HYDROXYACETANILIDE [J].

MYERS, TG ;

DIETZ, EC ;

ANDERSON, NL ;

KHAIRALLAH, EA ;

COHEN, SD ;

NELSON, SD .

CHEMICAL RESEARCH IN TOXICOLOGY, 1995, 8 (03) :403-413

[9] IN-GEL DIGESTION OF PROTEINS FOR INTERNAL SEQUENCE-ANALYSIS AFTER 1-DIMENSIONAL OR 2-DIMENSIONAL GEL-ELECTROPHORESIS [J].

ROSENFELD, J ;

CAPDEVIELLE, J ;

GUILLEMOT, JC ;

FERRARA, P .

ANALYTICAL BIOCHEMISTRY, 1992, 203 (01) :173-179

[10] 2-DIMENSIONAL ELECTROPHORETIC MAPPING OF HEPATIC AND RENAL STRESS PROTEINS [J].

WITZMANN, F ;

CLACK, J ;

FULTZ, C ;

JARNOT, B .

ELECTROPHORESIS, 1995, 16 (03) :451-459