DETERMINATION OF MEGESTROL-ACETATE AND CYPROTERONE-ACETATE IN SERUM OF PATIENTS WITH ADVANCED BREAST-CANCER BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：7

作者：

DIKKESCHEI, LD ^{[1
]}

WOLTHERS, BG ^{[1
]}

DERUYTERBUITENHUIS, AW ^{[1
]}

NAGEL, GT ^{[1
]}

SLEIJFER, DT ^{[1
]}

WILLEMSE, PHB ^{[1
]}

VANDERSLIK, W ^{[1
]}

机构：

[1] UNIV GRONINGEN HOSP,DEPT INTERNAL MED,DIV MED ONCOL,9700 RB GRONINGEN,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 529卷 / 01期

关键词：

D O I：

10.1016/S0378-4347(00)83815-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

A reversed-phase high-performance liquid chromatographic method with ultraviolet detection of megestrol acetate and cyproterone acetate in human sera is described. The proposed assay is linear up to 1400 ng/ml (r=0.999) and has a detection limit of 5 ng/ml. Recoveries of both compounds in spiked sera were ca. 95%; inter-assay coefficients of variation were 4.0 and 3.1% and intra-assay values were 1.3 and 1.4%, respectively. For validation of the method we also developed a gas chromatographic-mass spectrometric method for both steroids. The results obtained by the two methods showed good correlation: for megestrol acetate r=0.98, n=31, p 0.0001, and for cyproterone acetate r=0.94, n=0, p=0001. Large inter-individual differences in the serum concentrations of both substances were found in groups of patients with metastatic breast cancer receiving the same oral load of either steroid. © 1990.

引用

页码：145 / 154

页数：10

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