HUMAN BRAIN LECTIN - A SOLUBLE LECTIN THAT BINDS ACTIN

被引：34

作者：

JOUBERT, R

CARON, M

AVELLANAADALID, V

MORNET, D

BLADIER, D

机构：

[1] UNIV PARIS NORD,BIOCHIM & TECHNOL LAB,74 RUE MARCEL CACHIN,F-93012 BOBIGNY,FRANCE

[2] HOP AVICENNE,CENT BIOCHIM LAB,BOBIGNY,FRANCE

[3] FAC PHARM MONTPELLIER,INSERM,U300,F-34060 MONTPELLIER,FRANCE

来源：

JOURNAL OF NEUROCHEMISTRY | 1992年 / 58卷 / 01期

关键词：

ACTIN; AVIDIN; BIOTIN; CENTRAL NERVOUS SYSTEM; HUMAN BRAIN; LECTIN;

D O I：

10.1111/j.1471-4159.1992.tb09296.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A biotinylated probe was used for detection of endogenous ligands of a human brain lectin on blotted human brain soluble proteins. Of the various proteins from brain extract resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, five reacted with the biotinylated probe. After elimination of saccharidic moieties by periodic treatment of the same extract, a single band with M(r) approximately 43,000 was recognized by the lectin. This band was identified as actin using an anti-actin antibody. These results were confirmed by binding of biotinylated lectin to purified actin.

引用

页码：200 / 203

页数：4

共 21 条

[1] BIOTINYLATED DERIVATIVE OF A HUMAN BRAIN LECTIN - SYNTHESIS AND USE IN AFFINOBLOTTING FOR ENDOGENOUS LIGAND STUDIES [J].