CHROMATOGRAPHY IN DNA RADIOLABELING - HANDS-OFF AUTOMATION USING A ROBOTIC WORKSTATION

被引:1
作者
MISCHIATI, C
FERIOTTO, G
FIORENTINO, D
GAMBARI, R
机构
[1] UNIV FERRARA,DEPT BIOCHEM & MOLEC BIOL,I-44100 FERRARA,ITALY
[2] BECKMAN ANALYT,I-80126 NAPLES,ITALY
[3] UNIV FERRARA,CTR BIOTECHNOL,I-44100 FERRARA,ITALY
来源
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 664卷 / 02期
关键词
D O I
10.1016/0378-4347(94)00493-O
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The experiments described in the present paper were performed in order to determine whether the Biomek-1000 (Beckman Instruments, Fullerton, CA, USA) automated laboratory workstation can be used in a fully automated DNA labeling method followed by automated gravity-driven size exclusion purification of molecular probes. To this aim, we performed random oligodeoxyribonucleotide priming of a HIV-1 LTR probe that was used for molecular hybridization to Southern blotted polymerase chain reaction products. The results obtained demonstrate that the automatically labeled probe can be efficiently purified by automated and gravity-driven Sephadex G-50 chromatography, without any major changes in hybridization property. This robotic methodology can be used in several procedures employing radioisotope labeling.
引用
收藏
页码:303 / 310
页数:8
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