GLYCANS FROM STREPTOCOCCAL CELL-WALLS - MOLECULAR-STRUCTURE AND IMMUNOLOGICAL PROPERTIES OF AN ANTIGENIC GLYCAN FROM STREPTOCOCCUS-BOVIS

被引:23
作者
PAZUR, JH [1 ]
DROPKIN, DJ [1 ]
DREHER, KL [1 ]
FORSBERG, LS [1 ]
LOWMAN, CS [1 ]
机构
[1] PENN STATE UNIV, DEPT BIOCHEM & BIOPHYS, UNIVERSITY PK, PA 16802 USA
关键词
D O I
10.1016/0003-9861(76)90164-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The molecular structure and immunological properties of an antigenic glycan from the cell wall of S. bovis, strain C3, a member of the Group D streptococci, were determined by methylation analysis, periodate oxidation and hapten inhibition. The glycan is a tetraheteroglycan composed of 6-deoxy-L-talose, L-rhamnose, D-galactose and D-glucuronic acid. The sugar sequence and the types of glycosidic linkages of the glycan are a main chain of L-rhamnosyl-(1,3)-D-galactosyl-(1,2)-L-rhamnosyl-(1,3)-6-deoxy-L-talosyl(1,3)- units with D-glucuronosyl residues attached to position 4 of the 1st rhamnose of each repeating unit of the main chain. The D-glucuronic acid moiety is the primary immunodeterminant group of the glycan. On the basis of hapten inhibition data, it was concluded that the binding of the antigen to the antibody occurs at the hydroxyl groups at positions 2 and 3 and the carboxyl group at position 6 of the D-glucuronic acid moieties. The antigen was used to prepare [rabbit] antiserum with anti-glucuronic acid antibodies [for vaccines].
引用
收藏
页码:257 / 266
页数:10
相关论文
共 40 条
[1]  
ABDELAKHER M, 1952, J CHEM SOC, P3637
[2]   FETAL ANTIGENS IN CANCER [J].
ALEXANDER, P .
NATURE, 1972, 235 (5334) :137-+
[3]   GAS-LIQUID CHROMATOGRAPHY AND MASS SPECTROMETRY IN METHYLATION ANALYSIS OF POLYSACCHARIDES [J].
BJORNDAL, H ;
HELLERQV.CG ;
LINDBERG, B ;
SVENSSON, S .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 1970, 9 (08) :610-&
[4]  
CAPRA JD, 1975, IMMUNOGENETICS, V1, P417
[5]  
CHENG WC, 1973, J IMMUNOL, V111, P1677
[6]  
COLIGAN JE, 1975, J IMMUNOL, V114, P1654
[7]   *UBER GLYKOLESTER DER PEKTINSAURE .21. UBER PEKTINSTOFFE [J].
DEUEL, H .
HELVETICA CHIMICA ACTA, 1947, 30 (06) :1523-1534
[8]  
DISCHE Z, 1948, J BIOL CHEM, V175, P595
[9]  
DISCHE Z, 1947, J BIOL CHEM, V167, P189
[10]  
DROPKIN DJ, 1973, FED PROC, V32, P638