TREATMENT OF SEVERE MALE-FACTOR INFERTILITY WITH HIGH-CONCENTRATIONS OF MOTILE SPERM BY MICROINSEMINATION IN EMBRYO CRYOPRESERVATION STRAWS

被引：23

作者：

HAMMITT, DG

WALKER, DL

SYROP, CH

MILLER, TM

BENNETT, MR

机构：

[1] Department of Obstetrics and Gynecology, The University of Iowa Hospital and Clinics, Iowa City, 52242, Iowa

[2] Department of Urology, The University of Iowa Hospitals and Clinics, Iowa City, 52242, Iowa

来源：

JOURNAL OF IN VITRO FERTILIZATION AND EMBRYO TRANSFER | 1991年 / 8卷 / 02期

关键词：

MICROINSEMINATION; MALE-FACTOR INFERTILITY; OLIGO ASTHENOSPERMIA; INVITRO FERTILIZATION (IVF); PRONUCLEAR-STAGE EMBRYO TRANSFER (PROST); GAMETE MICROMANIPULATION;

D O I：

10.1007/BF01138663

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

A microinsemination technique was evaluated for treating our program's most severe cases of male-factor infertility. Oocytes were inseminated with high concentrations of motile sperm (1 to 9 x 10(6)/ml) in 10 to 150-mu-l within embryo cryopreservation straws. Fertilization was obtained in 20 of 29 (69%) couples treated by this technique. In the 15 patients in which only embryos generated from the straw technique were transferred, 7 clinical pregnancies resulted (46.7% per transfer). The implantation rate for couples receiving embryos from the straw technique only (12/58; 20.7%) compared favorably to that observed for other cases treated during this same time period with regular insemination techniques (111/766; 14.5%). Clinical pregnancy rates per transfer for IVF-ET, TET, and PROST were 33.0% (1/3), 0% (0/2), and 60.0% (6/10), respectively. The percentage of polyploidic embryos was significantly lower (P < 0.0001) for male-factor patients treated by the straw technique with high sperm concentrations than for non-male-factor patients treated during this same time period with standard sperm concentrations. Normal births have resulted from straw inseminations with 3.4 x 10(6) and ongoing pregnancies with 5.0 x 10(6) motile sperm/ml. The results of this study suggest that some cases of male-factor infertility can be successfully treated by insemination with high concentrations of motile sperm in embryo cryopreservation straws. A technique of centrifuging sperm in straws was also developed to concentrate the entire fraction of washed sperm into 10-mu-l. Further development of this technique may allow treatment of more severe cases of oligo/asthenospermia by microinsemination with high concentrations of motile sperm than is presently possible with standard washing techniques.

引用

页码：101 / 110

页数：10

共 31 条

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