RAPID GENERATION OF DIRECTED AND UNMARKED DELETIONS IN XANTHOMONAS

被引：28

作者：

KAMOUN, S ^{[1
]}

TOLA, E ^{[1
]}

KAMDAR, H ^{[1
]}

KADO, CI ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,DEPT PLANT PATHOL,DAVIS,CA 95616

来源：

MOLECULAR MICROBIOLOGY | 1992年 / 6卷 / 06期

关键词：

D O I：

10.1111/j.1365-2958.1992.tb01531.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have devised a rapid four-step procedure for the generation of directed and unmarked chromosomal deletions in bacteria, based on the use of a novel cloning vector containing the Bacillus subtilis sacB gene that encodes levansucrase and confers sucrose sensitivity, which can be used for counter-selection. Using this technique, we describe the construction of a 6.5 kb directed and unmarked deletion in a phytopathogenicity region of the chromosome in Xanthomonas campestris. This procedure allows rapid and easy transfer of a wide variety of mutant allelic DNA to the bacterial chromosome, and should be adaptable to various bacteria besides Xanthomonas spp.

引用

页码：809 / 816

页数：8

共 37 条

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