INTRACELLULAR GLYCOGEN AND TREHALOSE CONTENTS AS PREDICTORS OF YEAST VIABILITY

被引：46

作者：

SLAUGHTER, JC

NOMURA, T

机构：

[1] International Centre for Brewing and Distilling, Department of Biological Sciences, Heriot Watt University, Edinburgh, Scotland

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1992年 / 14卷 / 01期

关键词：

GLYCOGEN; TREHALOSE; VIABILITY; YEAST; SACCHAROMYCES-CEREVISIAE; FERMENTATION;

D O I：

10.1016/0141-0229(92)90028-M

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The use of intracellular glycogen content to determine the amount of yeast which must be added to start a brewery fermentation, as suggested by Quain and Tubb in 1982, has met with mixed success. We have studied the changes in cell viability, as determined by methylene blue staining, along with the intracellular concentration of trehalose and acid- and alkali-soluble glycogen, under a number of experimental conditions in the laboratory: different temperatures, acidification of the medium, and addition of cycloheximide to the medium. The results indicate that while there can be a zone of correlation between viability and the acid-soluble glycogen or trehalose content of the cells, this relationship is specific to the experimental conditions and the storage carbohydrate content of cells cannot be used to predict cell viability without knowledge of the conditions of yeast storage.

引用

页码：64 / 67

页数：4