CONTINUOUS BUFFER EXCHANGE OF COLUMN CHROMATOGRAPHIC ELUATES USING A HOLLOW-FIBER MEMBRANE MODULE

被引：11

作者：

KAUFMANN, M

SCHWARZ, T

BARTHOLMES, P

机构：

[1] Institute of Biochemistry, University of Witten/Herdecke, W-5810 Witten

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 639卷 / 01期

关键词：

D O I：

10.1016/0021-9673(93)83085-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method was developed to remove continuously the concentration gradient of any low-molecular-mass substance in the eluate during adsorption liquid column chromatography. For this purpose, a hollow-fibre membrane module (HFMM) was directly connected to the outlet of the chromatographic column and on-line buffer exchange was achieved by cross-flow ultradialysis. It could be demonstrated that the HFMM when used during liquid chromatography has some additional advantages. It is an ideal tool to concentrate continuously column eluates by dialysis against polyethylene glycol solutions. Moreover, it is also possible to detect specifically enzyme activities on-line using an HFMM.

引用

页码：33 / 39

页数：7

共 21 条

[1] MECHANISM OF RECONSTITUTION OF THE APO-BETA2 SUBUNIT AND THE ALPHA-2APO-BETA2 COMPLEX OF TRYPTOPHAN SYNTHASE WITH PYRIDOXAL 5'-PHOSPHATE - KINETIC-STUDIES [J].