PRODUCTION, CHARACTERIZATION AND USE OF MONOCLONAL-ANTIBODIES TO HUMAN INTERLEUKIN-5 IN AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY

被引：37

作者：

MCNAMEE, LA

FATTAH, DI

BAKER, TJ

BAINS, SK

HISSEY, PH

机构：

[1] Division of Cellular and Molecular Sciences, Glaxo Group Research Ltd., Greenford, Middx.

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 141卷 / 01期

关键词：

INTERLEUKIN-5; ANTI-HUMAN; MONOCLONAL ANTIBODY; ELISA;

D O I：

10.1016/0022-1759(91)90212-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two mouse monoclonal antibodies (Mabs) against recombinant human interleukin-5(rhIL-5) have been produced, characterised and purified. Both are IgG1 antibodies and neutralised the activity of rhIL-5 in the B13 assay. Neither Mab cross-reacted with mouse IL-5. A two-site sandwich enzyme-linked immunosorbent assay (ELISA) was developed with different combinations of the mouse Mabs and also a rat anti-mouse IL-5 Mab, TRFK5, which also has activity against rhIL-5. The most sensitive assay, with a lower detection limit of 0.5 ng/ml IL-5, used TRFK5 as the capture antibody and the mouse anti-human IL-5 Mab as second antibody. The sensitivity of this assay was increased by an enhanced chemiluminescent reagent and resulted in a lower limit of detection around 40 pg/ml IL-5.

引用

页码：81 / 88

页数：8

共 24 条

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