IDENTIFICATION OF ENTEROHEMORRHAGIC ESCHERICHIA-COLI BY MEANS OF THEIR PRODUCTION OF ENTEROHEMOLYSIN

被引:42
作者
BETTELHEIM, KA
机构
[1] Biomedical Reference Laboratory, VIDRL, Fairfield Hospital, Fairfield, Victoria
来源
JOURNAL OF APPLIED BACTERIOLOGY | 1995年 / 79卷 / 02期
关键词
D O I
10.1111/j.1365-2672.1995.tb00932.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
With increasing interest in verocytotoxigenic Escherichia roll (VTEC) being associated with both human and animal infection, a simple system of rapidly identifying the majority of VTEC has been devised. This depends on the fact that most VTEC produce enterohaemolysin, which is rarely produced by non-VTEC. By employing two media, traditional sheep blood agar (SEA) and washed sheep blood agar supplemented with calcium (WSBA-Ca), enterohaemolysin-producing strains can be easily differentiated from other E. coli enabling most VTEC types, including ones belonging to serogroups other than O157, to be isolated.
引用
收藏
页码:178 / 180
页数:3
相关论文
共 16 条
  • [1] Acheson D.W.K., Keusch G.T., Lightowlers M., Donohue-Rolfe A., Enzyme‐linked immunosorbent assay for Shiga toxin and Shiga‐like toxin II using P<sub>1</sub> glycoprotein from hydatid cysts, Journal of Infectious Diseases, 127, pp. 1145-1150, (1990)
  • [2] Bettelheim K.A., Thompson C.J., New method of serotyping Escherichia coli: implementation and varification, Journal of Clinical Microbiology, 25, pp. 781-786, (1987)
  • [3] Beutin L., The different hemolysins of Escherichia coli, Medical Microbiology and Immunology, 180, pp. 167-182, (1991)
  • [4] Beutin L., Montenegro M.A., Orskov I., Orskov F., Prada J., Zimmermann S., Stephan R., Close association of verocytotoxin (Shiga‐like Toxin) production with entero‐hemolysin production in strains of Escherchia coli, Journal of Clinical Microbiology, 27, pp. 2559-2564, (1989)
  • [5] Beutin L., Aleksic' S., Zimmermann S., Gleier K., Virulence factors and phenotypical traits of verotoxigenc strains of Escherichia coli isolated from human patients in Germany, Medical Microbiology and Immunology, 138, pp. 13-21, (1994)
  • [6] Chandler M.E., Bettelheim K.A., A rapid method of identifying Escherichia coli“H” antigens. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene I. Abteilung Originale. A, 129, pp. 74-79, (1974)
  • [7] Gunzburg S., Gracey M., Forbes D., Hewitt I., Bettelheim K.A., Haemolytic‐uraemic syndrome and verotoxigenic Esch. coli, Medical Journal of Australia, 149, pp. 54-55, (1988)
  • [8] Gunzer F., Bohm H., Russmann H., Bitzan M., Aleksic S., Karch H., Molecular detection of sorbitol‐fermenting Escherichia coli O157 in patients with hemolytic‐uremic syndrome, Journal of Clinical Microbiology, 30, pp. 1807-1810, (1992)
  • [9] Karch H., Meyer T., Russmann H., Heesemann J., Frequent loss of Shiga‐like toxin genes in clinical isolates of Escherichia coli upon subcultivation, Infection and Immunity, 60, pp. 3464-3467, (1992)
  • [10] Konowalchuk J., Speirs J.L., Stavric S., Vero response to a cytotoxin of Escherichia coli, Infection and Immunity, 18, pp. 775-779, (1977)