MOLECULAR-CLONING OF THE HUMAN HEPATITIS-C VIRUS GENOME FROM JAPANESE PATIENTS WITH NON-A, NON-B HEPATITIS

被引：1143

作者：

KATO, N

HIJIKATA, M

OOTSUYAMA, Y

NAKAGAWA, M

OHKOSHI, S

SUGIMURA, T

SHIMOTOHNO, K

机构：

[1] Virology Division, Natl. Cancer Ctr. Research Institute, Chuo-ku, Tokyo 104, 5-1-1, Tsukiji

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 24期

关键词：

Gene structure; Nucleotide sequence; RNA virus;

D O I：

10.1073/pnas.87.24.9524

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The nucleotide sequence of the Japanese type of hepatitis C virus (HCV-J) genome, consisting of 9413 nucleotides, was determined by analyses of cDNA clones from plasma specimens from Japanese patients with chronic hepatitis. HCV-J genome contains a long open reading frame that can encode a sequence of 3010 amino acid residues. Comparison of HCV-J with the American isolate of HCV showed 22.6% difference in nucleotide sequence and 15.1% difference in amino acid sequence. Thus HCV-J and the American isolate of HCV are probably different subtypes of HCV. The relationship of HCV-J with other animal RNA virus families and the putative organization of the HCV-J genome are discussed. (.

引用

页码：9524 / 9528

页数：5

共 31 条

[1] SCREENING GAMMAGT RECOMBINANT CLONES BY HYBRIDIZATION TO SINGLE PLAQUES INSITU [J].