AN EVALUATION OF THE ESCHERICHIA-COLI K-12 UVRB/RECA DNA-REPAIR HOST-MEDIATED ASSAY .1. INVITRO SENSITIVITY OF THE BACTERIA TO 61 COMPOUNDS

被引:28
作者
HELLMER, L
BOLCSFOLDI, G
机构
[1] AB Astra, Safety Assessment, Södertälje
来源
MUTATION RESEARCH | 1992年 / 272卷 / 02期
关键词
ESCHERICHIA-COLI K-12; DNA REPAIR TEST INVITRO;
D O I
10.1016/0165-1161(92)90043-L
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A differential DNA repair test was evaluated in vitro, using derivatives of E. coli K-12 343/113 with the genotype uvrB-/recA- and uvrB+/recA+. The aim of this study was to characterize the sensitivity of the assay to different compounds in vitro and thereby provide information on the usefulness of this end-point as an indicator of genotoxicity in a host-mediated assay. Sixty-one compounds from diverse chemical groups were tested and of these 32 gave a positive result. The results obtained were compared with results from the Ames test and were in agreement for 49 out of the 61 compounds tested. Chemicals that were detected in this test but negative in the Ames test were 4-aminophenol, catechol, diethylstilbestrol, thioacetamide and thiourea. Seven of the compounds tested gave a negative result in E. coli but were positive in Salmonella. These were 4-aminobiphenyl, benzo[a]pyrene, cyclophosphamide, 1-naphthylamine, N-nitrosobutylpropylamine, quinoline and 2-toluidine. The performance of the in vitro test and reasons for the discrepant results with the Ames test are discussed. The overall concordance between the two tests was about 80%. On the basis of these results we consider these bacterial strains, and differential DNA repair as an end-point, to be sufficiently accurate as an indicator of genotoxicity in vitro and thereby also in vivo.
引用
收藏
页码:145 / 160
页数:16
相关论文
共 46 条
[1]   CHROMOSOME-ABERRATIONS AND SISTER CHROMATID EXCHANGES IN CHINESE-HAMSTER CELLS EXPOSED TO VARIOUS CHEMICALS [J].
ABE, S ;
SASAKI, M .
JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1977, 58 (06) :1635-1641
[2]   IMPROVED BACTERIAL TEST SYSTEM FOR DETECTION AND CLASSIFICATION OF MUTAGENS AND CARCINOGENS [J].
AMES, BN ;
LEE, FD ;
DURSTON, WE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (03) :782-786
[3]   DEFINITIVE RELATIONSHIPS AMONG CHEMICAL-STRUCTURE, CARCINOGENICITY AND MUTAGENICITY FOR 301 CHEMICALS TESTED BY THE UNITED-STATES NTP [J].
ASHBY, J ;
TENNANT, RW .
MUTATION RESEARCH, 1991, 257 (03) :229-306
[4]  
ASHBY J, 1981, EVALUATION SHORT TER, P33
[5]  
ASHBY J, 1985, PROGR MUTATION RES, V5
[6]  
Bridges B. A., 1981, EVALUATION SHORT TER, P49
[7]  
BROOKES P, 1981, PROGR MUTATION RES, V1, P77
[8]   MUTAGENICITY OF QUINONES - PATHWAYS OF METABOLIC-ACTIVATION AND DETOXIFICATION [J].
CHESIS, PL ;
LEVIN, DE ;
SMITH, MT ;
ERNSTER, L ;
AMES, BN .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (06) :1696-1700
[9]   GENOTOXIC ACTIVITY AND POTENCY OF 135 COMPOUNDS IN THE AMES REVERSION TEST AND IN A BACTERIAL DNA-REPAIR TEST [J].
DEFLORA, S ;
ZANACCHI, P ;
CAMOIRANO, A ;
BENNICELLI, C ;
BADOLATI, GS .
MUTATION RESEARCH, 1984, 133 (03) :161-198
[10]   REPRODUCIBILITY OF MICROBIAL MUTAGENICITY ASSAYS .2. TESTING OF CARCINOGENS AND NONCARCINOGENS IN SALMONELLA-TYPHIMURIUM AND ESCHERICHIA-COLI [J].
DUNKEL, VC ;
ZEIGER, E ;
BRUSICK, D ;
MCCOY, E ;
MCGREGOR, D ;
MORTELMANS, K ;
ROSENKRANZ, HS ;
SIMMON, VF .
ENVIRONMENTAL MUTAGENESIS, 1985, 7 :1-248