CHARACTERIZATION OF A MONOCLONAL-ANTIBODY AGAINST LATEX PROTEIN ASSOCIATED WITH LATEX ALLERGY

被引：21

作者：

KURUP, VP ^{[1
]}

KUMAR, A ^{[1
]}

KELLY, KJ ^{[1
]}

FINK, JN ^{[1
]}

机构：

[1] VET AFFAIRS MED CTR,RES SERV,MILWAUKEE,WI

来源：

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY | 1993年 / 92卷 / 05期

关键词：

MONOCLONAL ANTIBODY; LATEX PROTEIN; LATEX ALLERGY; RUBBER PLANT EXTRACT;

D O I：

10.1016/0091-6749(93)90005-Z

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Several hybridomas were produced against antigens extracted from the latex sap of Hevea brasiliensis. One of the monoclonal antibodies (LA-E3) reacted with antigens demonstrating binding to patient sera on crossed enzyme immunoelectrophoresis. This monoclonal reacted with 2 of 10 glove extracts studied and with both Malaysian and Indian law extracts. The antigens purified with monoclonal affinity chromatography demonstrated specific IgE in the sera of patients with later allergy as determined by ELISA. This monoclonal antibody can thus be utilized to obtain reliable antigens useful in the diagnosis of law sensitivity, although additional antigens will likely be necessary to enhance sensitivity and specificity.

引用

页码：638 / 643

页数：6

共 26 条

[1] SURGICAL LATEX GLOVE ALLERGY - CHARACTERIZATION OF RUBBER PROTEIN ALLERGENS BY IMMUNOBLOTTING [J].