TRISOMY-12 IN CHRONIC LYMPHOCYTIC-LEUKEMIA AND HAIRY-CELL LEUKEMIA - A CYTOGENETIC AND INTERPHASE CYTOGENETIC STUDY

被引：20

作者：

CUNEO, A

BIGONI, R

BALBONI, M

CARLI, MG

PIVA, N

FAGIOLI, F

LATORRACA, A

WLODARSKA, I

VANDENBERGHE, H

CASTOLDI, G

机构：

[1] Institute of Hematology, University of Ferrara, Ferrara

[2] Centre for Human Genetics, University of Leuven, Leuven

来源：

LEUKEMIA & LYMPHOMA | 1994年 / 15卷 / 1-2期

关键词：

TRISOMY; 12; FISH; CLL; HCL;

D O I：

10.3109/10428199409051693

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Fluorescent in situ hybridization (FISH) with a chromosome 12-specific pericentromeric probe was performed in 42 patients with B-cell chronic lymphocytic leukemia (CLL) and in 10 patients with hairy cell leukemia (HCL). In all cases, a normal karyotype in more than 10 metaphase cells was obtained by conventional chromosome study. FISH documented that 6/42 patients with CLL in fact had trisomy 12 in 15-49% interphase cells. Sequential FISH studies were performed in 2 cases, showing an increase of percentage of trisomic cells over a 2-month to 4-year period. Two out of 10 patients with HCL, one of whom had morphologic features consistent with a diagnosis of HCL variant, showed 5.5 and 10% interphase nuclei with three fluorescent signals, a finding suggestive of the presence of trisomy 12. Combined immunophenotyping and FISH staining in these patients with HCL documented that trisomic cells were CD11c-positive, CD13-negative, and CD2-negative. We conclude that FISH is a sensitive technique allowing for the detection of trisomy 12 in a fraction of cytogenetically normal patients affected with CLL and HCL.

引用

页码：167 / 172

页数：6

共 24 条

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