A NEW DERIVATIVE FOR IMPROVED GLC SEPARATION OF MANNOSE, GALACTOSE AND GLUCOSE

被引:5
作者
ALHAZMI, MI [1 ]
STAUFFER, KR [1 ]
OLNEY, C [1 ]
机构
[1] UNIV RHODE ISL, DEPT FOOD SCI & TECHNOL NUTR & DIETET, KINGSTON, RI 02881 USA
关键词
D O I
10.1007/BF02265481
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The procedure involving oxime formation followed by silylation for the analysis of standard hexoses by GC resulted in unseparated peaks for D-glucose, D-mannose and D-galactose. This procedure was therefore modified to obtain clear separation of hexoses. STOX (hydroxylamine containing phenyl-.beta.-D-glucopyranoside as the internal standard) was mixed with trimethylsilylimidazol or hexamethyldisilazane, and then added to a standard sugar mixture containing D-glucose, D-galactose and D-mannose. The mixture was analyzed by GC with OV-17/OV-22 as the stationary phase, resulting in 3 distinct peaks.
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页码:403 / 406
页数:4
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