TRANSCRIPTIONAL ACTIVATION OF THE RAT GLUCAGON GENE BY THE CYCLIC AMP-RESPONSIVE ELEMENT IN PANCREATIC-ISLET CELLS

被引：71

作者：

KNEPEL, W ^{[1
]}

CHAFITZ, J ^{[1
]}

HABENER, JF ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,HOWARD HUGHES MED INST,BOSTON,MA 02115

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 12期

关键词：

D O I：

10.1128/MCB.10.12.6799

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 5'-flanking region of the rat glucagon gene contains, from nucleotides -291 to -298, a sequence (TGA CGTCA) which mediates cyclic AMP (cAMP) responsiveness in several genes (cAMP-responsive element [CRE]). However, because of nonpermissive bases surrounding in CRE octamer, the glucagon CRE does not confer cAMP responsiveness to an inert heterologous promoter in placental JEG cells that do not express the glucagon gene. This report describes transient transfection experiments with glucagon-reporter fusion genes that show that glucagon gene expression is activated by cAMP-dependent protein kinase A in a glucagon-expressing pancreatic islet cell line. This activation is mediated through the glucagon CRE.

引用

页码：6799 / 6804

页数：6

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