RAPID PULSED-FIELD CAPILLARY ELECTROPHORETIC SEPARATION OF MEGABASE NUCLEIC-ACIDS

被引：68

作者：

KIM, YS ^{[1
]}

MORRIS, MD ^{[1
]}

机构：

[1] UNIV MICHIGAN,DEPT CHEM,ANN ARBOR,MI 48109

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 05期

关键词：

D O I：

10.1021/ac00101a002

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Pulsed field capillary electrophoresis in ultradilute solutions of sieving polymers has been used to separate nucleic acid fragments as long as 1.6 million base pairs. Hydroxyethyl cellulose solutions are used for separations in the size range 8000-50 000 base pairs. Longer chain nucleic acids are separated in mixed hydroxyethyl cellulose/poly(ethylene oxide) solutions. Separations are rapid (12-13 min).

引用

页码：784 / 786

页数：3

共 18 条

[1] A TRANSIENT ENTANGLEMENT COUPLING MECHANISM FOR DNA SEPARATION BY CAPILLARY ELECTROPHORESIS IN ULTRADILUTE POLYMER-SOLUTIONS
BARRON, AE
BLANCH, HW
SOANE, DS
[J]. ELECTROPHORESIS, 1994, 15 (05) : 597 - 615
[2] CAPILLARY ELECTROPHORESIS IN AGAROSE SOLUTIONS - EXTENSION OF SIZE SEPARATIONS TO DNA OF 12-KB IN LENGTH
BOCEK, P
CHRAMBACH, A
[J]. ELECTROPHORESIS, 1992, 13 (1-2) : 31 - 34
[3] IMPROVED SEPARATION OF NUCLEIC-ACIDS WITH ANALYTE VELOCITY MODULATION CAPILLARY ELECTROPHORESIS
DEMANA, T
LANAN, M
MORRIS, MD
[J]. ANALYTICAL CHEMISTRY, 1991, 63 (23) : 2795 - 2797
[4] A STABLE DOUBLE-STRANDED DNA ETHIDIUM HOMODIMER COMPLEX - APPLICATION TO PICOGRAM FLUORESCENCE DETECTION OF DNA IN AGAROSE GELS
GLAZER, AN
PECK, K
MATHIES, RA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (10) : 3851 - 3855
[5] CAPILLARY ELECTROPHORESIS OF DNA IN ENTANGLED POLYMER-SOLUTIONS
GROSSMAN, PD
SOANE, DS
[J]. JOURNAL OF CHROMATOGRAPHY, 1991, 559 (1-2): : 257 - 266
[6] CAPILLARY ZONE ELECTROPHORESIS OF LARGE DNA
GUSZCZYNSKI, T
PULYAEVA, H
TIETZ, D
GARNER, MM
CHRAMBACH, A
[J]. ELECTROPHORESIS, 1993, 14 (5-6) : 523 - 530
[7] SEPARATION OF DNA RESTRICTION FRAGMENTS BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS WITH LOW AND ZERO CROSS-LINKED POLYACRYLAMIDE USING CONTINUOUS AND PULSED ELECTRIC-FIELDS
HEIGER, DN
COHEN, AS
KARGER, BL
[J]. JOURNAL OF CHROMATOGRAPHY, 1990, 516 (01): : 33 - 48
[8] HIGH-PERFORMANCE ELECTROPHORESIS - ELIMINATION OF ELECTROENDOSMOSIS AND SOLUTE ADSORPTION
HJERTEN, S
[J]. JOURNAL OF CHROMATOGRAPHY, 1985, 347 (02): : 191 - 198
[9] PULSED-FIELD CAPILLARY ELECTROPHORESIS OF MULTIKILOBASE LENGTH NUCLEIC-ACIDS IN DILUTE METHYL CELLULOSE SOLUTIONS
KIM, Y
MORRIS, MD
[J]. ANALYTICAL CHEMISTRY, 1994, 66 (19) : 3081 - 3085
[10] VARIABLE FREQUENCY-MODULATION IN DNA SEPARATIONS USING FIELD-INVERSION CAPILLARY GEL-ELECTROPHORESIS
NAVIN, MJ
RAPP, TL
MORRIS, MD
[J]. ANALYTICAL CHEMISTRY, 1994, 66 (07) : 1179 - 1182

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