PARTIAL-PURIFICATION OF THE EXTRACELLULAR PROTEOLYTIC SYSTEM OF AEROMONAS-HYDROPHILA LP50 - COMPARISON OF CHROMATOGRAPHIC AND ELECTROPHORETIC METHODS

被引：3

作者：

DENIS, F ^{[1
]}

VEILLETPONCET, L ^{[1
]}

机构：

[1] INST NATL POLYTECH LORRAINE, ECOLE NATL SUPER AGRON & IND ALIMENTAIRES, MICROBIOL ALIMENTAIRE LAB, F-54000 NANCY, FRANCE

来源：

ANNALES DE MICROBIOLOGIE | 1984年 / A135卷 / 02期

关键词：

D O I：

10.1016/S0769-2609(84)80004-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A complex extracellular proteolytic system was produced by A. hydrophila LP50 on glucose-polypeptone medium. Partial purification of this system was accomplished by (NH4)2SO4 precipitation, acetone precipitation, gel filtration on Sephacryl-S200 and chromatography on DEAE-Sephacel. Every stage was controlled by electrophoresis. This proteolytic system consisted of 3 aminopeptidasic and 2 endopeptidasic components.

引用

页码：219 / 227

页数：9

共 20 条

[1] EXTRACELLULAR VIRULENCE FACTORS OF AEROMONAS-HYDROPHILA IN FISH INFECTIONS [J].