CHARACTERIZATION OF SIGNALS PROMOTING GENE-EXPRESSION ON THE STAPHYLOCOCCUS-AUREUS PLASMID PUB110 AND DEVELOPMENT OF A GRAM-POSITIVE EXPRESSION VECTOR SYSTEM

被引:45
作者
ZYPRIAN, E [1 ]
MATZURA, H [1 ]
机构
[1] UNIV HEIDELBERG, NEUENHEIMER FELD 230, D-6900 HEIDELBERG, GERMANY
来源
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY | 1986年 / 5卷 / 03期
关键词
D O I
10.1089/dna.1986.5.219
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The transcriptional and translational initiation signals of a portion of the Staphylococcus aureus plasmid pUB110 were analyzed. An Mbo I-Pvu II fragment was sequenced and the site of transcriptional initiation was determined by in vitro mapping. To convert the plasmid into a cloning vector, a multilinker was introduced in different positions relative to a detected reading frame. The Gram-negative .beta.-galactosidase gene and the Gram-positive chloramphenicol acetyltransferase (cat) gene were fused and the level of expression was determined in Bacillus subtilis. Hybrid proteins consisting of corresponding CAT polypeptides were produced in each translational reading frame. Therefore this vector system can be used to express cloned DNA in the Gram-positive host Bacillus subtilis. Furthermore a derived lacZ fusion plasmid may be used for rapid screening of inserts.
引用
收藏
页码:219 / 225
页数:7
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