3-DIMENSIONAL STRUCTURE OF THE NICKEL-CONTAINING HYDROGENASE FROM THIOCAPSA-ROSEOPERSICINA

被引:20
作者
SHERMAN, MB
ORLOVA, EV
SMIRNOVA, EA
HOVMOLLER, S
ZORIN, NA
机构
[1] ACAD SCI USSR,INST CRISTALLOG,MOSCOW V-71,USSR
[2] ACAD SCI USSR,INST SOIL SCI & PHOTOSYNTH,PUSHCHINO,USSR
[3] UNIV STOCKHOLM,S-10691 STOCKHOLM,SWEDEN
关键词
D O I
10.1128/jb.173.8.2576-2580.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped complex containing six each of the large (62-kDa) and small (26-kDa) subunits. The complex is very open, with six well-separated dumbbell-shaped masses surrounding a large cylindrical hole. Each dumbbell is interpreted as consisting of one large and one small subunit.
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页码:2576 / 2580
页数:5
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