REGIONAL MAPPING OF 22 MICROCLONES AROUND THE ADENOMATOUS POLYPOSIS-COLI (APC) LOCUS ON CHROMOSOME-5Q

被引：5

作者：

HAMPTON, GM

HOWE, C

LEUTERITZ, G

THOMAS, H

BODMER, WF

SOLOMON, E

BALLHAUSEN, WG

机构：

[1] UNIV ERLANGEN NURNBERG,INST HUMAN GENET,SCHWABACHANLAGE 10,W-8520 ERLANGEN,GERMANY

[2] IMPERIAL CANC RES FUND,DIRECTORS LAB,LONDON WC2A 3PX,ENGLAND

[3] IMPERIAL CANC RES FUND,SOMAT CELL GENET LAB,LONDON WC2A 3PX,ENGLAND

来源：

HUMAN GENETICS | 1991年 / 88卷 / 01期

关键词：

D O I：

10.1007/BF00204940

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A previously described genomic library constructed from microdissected DNA has been used to generate a large number of probes around the adenomatous polyposis coli (APC) gene at 5q22. A total of 202 clones were hybridised directly onto a somatic cell hybrid panel containing two APC-related interstitial deletions. Of 75 microclones that gave clear hybridisation signals, 22 independent clones mapped into the region common to both deletions. In addition, 4/22 of the markers are conserved in rodent DNA. These clones should provide a valuable resource for screening cDNA libraries and cloning the DNA around the APC gene in yeast artificial chromosomes.

引用

页码：112 / 114

页数：3

共 18 条

[1]

[Anonymous], 1985, INT SYSTEM HUMAN CYT

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