MEASUREMENT OF CEFTAZIDIME ARGININE IN AQUEOUS-SOLUTION BY HPLC

Ceftazidime is used widely for the treatment of infections caused by gram-negative microorganisms. Ceftazidime arginine is its newest formulation available in the U.S.. It is reconstituted and stored in plastic syringes. A high-performance liquid chromatographic (HPLC) method was developed to measure ceftazidime arginine in aqueous solution to determine its stability during storage in plastic syringes. The chromatographic separation was achieved using an ultrasphere ODS column and mobile phase containing 89% 0.01 M ammonium acetate buffer and 11% methanol, at a detection wave length of 254 nm. The retention time of ceftazidime arginine was about 4.27 minutes. The accuracy ranged from 98.6 to 99.8% and the coefficient of variation was less than 1%. The technique was used to determine the stability of ceftazidime arginine in sterile water for injection during storage for 24 hours in plastic syringes.