EXPRESSION OF A YEAST GENE CAN BE BLOCKED BY INSERTION OF SHORT YEAST DNA FRAGMENTS BETWEEN A UAS AND THE TATA BOX

被引：6

作者：

VINCENT, O ^{[1
]}

GANCEDO, JM ^{[1
]}

机构：

[1] CSIC,INST INVEST BIOMED,E-28029 MADRID,SPAIN

来源：

CURRENT GENETICS | 1995年 / 27卷 / 04期

关键词：

SACCHAROMYCES CEREVISIAE; UAS; FBP1; TRANSCRIPTION;

D O I：

10.1007/BF00352109

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have constructed a plasmid, pOV10, which facilitates the introduction of putative upstream activating sequences (UAS) or upstream repressing sequences (URS) from yeast genes into plasmids containing CYC1-lacZ fusions. We have observed that the insertion of yeast sequences from 155 to 195 bp between the UAS and the TATA box of a CYC1-lacZ fusion gene can block beta-galactosidase expression. It is suggested that this block is related to the formation of nucleosomes on the DNA.

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页码：387 / 389

页数：3

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