POSSIBLE INVOLVEMENT OF ACTIVE OXYGEN SPECIES IN SELENITE TOXICITY IN ISOLATED RAT HEPATOCYTES

被引：57

作者：

KITAHARA, J ^{[1
]}

SEKO, Y ^{[1
]}

IMURA, N ^{[1
]}

机构：

[1] KITASATO UNIV,SCH PHARMACEUT SCI,DEPT PUBL HLTH,MINATO KU,TOKYO 108,JAPAN

来源：

ARCHIVES OF TOXICOLOGY | 1993年 / 67卷 / 07期

关键词：

SELENITE TOXICITY; SUPEROXIDE ANION; ACTIVE OXYGEN; RAT HEPATOCYTES;

D O I：

10.1007/BF01969921

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Mechanisms of selenite cytotoxicity were examined using isolated rat hepatocytes. When selenite was added to a suspension of rat hepatocytes, intracellular reduced glutathione (GSH) was decreased and the oxygen consumption rate was increased. Subsequently, thiobarbituric acid-reactive substances (TBA-RS) and lactate dehydrogenase (LDH) leakage were increased. A ferric iron chelator, desferrioxamine (DF), and a synthetic superoxide dismutase (SOD) mimic, desferrioxamine manganese (DFMn), reduced the selenite toxicity. These results suggest that superoxide anion and its reactive metabolites such as the hydroxyl radical may be involved in the cytotoxicity of selenite.

引用

页码：497 / 501

页数：5

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