X-RAY-ANALYSIS REVEALS CONFORMATIONAL ADAPTATION OF THE LINKER IN FUNCTIONAL CALMODULIN MUTANTS

被引：24

作者：

MEADOR, WE

GEORGE, SE

MEANS, AR

QUIOCHO, FA

机构：

[1] BAYLOR COLL MED,HOWARD HUGHES MED INST,HOUSTON,TX 77030

[2] BAYLOR COLL MED,DEPT BIOCHEM,HOUSTON,TX 77030

[3] DUKE UNIV,SCH MED,DEPT MED,DURHAM,NC 27719

[4] DUKE UNIV,SCH MED,DEPT PHARMACOL,DURHAM,NC 27719

来源：

NATURE STRUCTURAL BIOLOGY | 1995年 / 2卷 / 11期

关键词：

D O I：

10.1038/nsb1195-943

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystal structures of target peptide bound Ca2+-calmodulin linker helix mutants with the helix shortened by two or th ree residues, or replaced by the longer troponin C central helix, show that the major calmodulin-target structure is preserved at the expense of linker conformation.

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页码：943 / 945

页数：3

相关论文

共 17 条

[1] STRUCTURE OF CALMODULIN REFINED AT 2.2 A RESOLUTION [J].

BABU, YS ;

BUGG, CE ;

COOK, WJ .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 204 (01) :191-204

[2] BACKBONE DYNAMICS OF CALMODULIN STUDIED BY N-15 RELAXATION USING INVERSE DETECTED 2-DIMENSIONAL NMR-SPECTROSCOPY - THE CENTRAL HELIX IS FLEXIBLE [J].

BARBATO, G ;

IKURA, M ;

KAY, LE ;

PASTOR, RW ;

BAX, A .

BIOCHEMISTRY, 1992, 31 (23) :5269-5278

[3] 4CA2+.TROPONIN-C FORMS DIMERS IN SOLUTION AT NEUTRAL PH THAT DISSOCIATE UPON BINDING VARIOUS PEPTIDES - SMALL-ANGLE X-RAY-SCATTERING STUDIES OF PEPTIDE-INDUCED STRUCTURAL-CHANGES [J].

BLECHNER, SL ;

OLAH, GA ;

STRYNADKA, NCJ ;

HODGES, RS ;

TREWHELLA, J .

BIOCHEMISTRY, 1992, 31 (46) :11326-11334

[4]

BRUNGER A, XPLOR 3 1 SYSTEM CRY

[5] CALMODULIN STRUCTURE REFINED AT 1.7 ANGSTROM RESOLUTION [J].

CHATTOPADHYAYA, R ;

MEADOR, WE ;

MEANS, AR ;

QUIOCHO, FA .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 228 (04) :1177-1192

[6]

GEORGE SE, 1993, J BIOL CHEM, V268, P25213

[7]

GULATI J, 1993, J BIOL CHEM, V268, P11685

[8] SOLUTION STRUCTURE OF A CALMODULIN-TARGET PEPTIDE COMPLEX BY MULTIDIMENSIONAL NMR [J].

IKURA, M ;

CLORE, GM ;

GRONENBORN, AM ;

ZHU, G ;

KLEE, CB ;

BAX, A .

SCIENCE, 1992, 256 (5057) :632-638

[9] MODULATION OF CALMODULIN PLASTICITY IN MOLECULAR RECOGNITION ON THE BASIS OF X-RAY STRUCTURES [J].

MEADOR, WE ;

MEANS, AR ;

QUIOCHO, FA .

SCIENCE, 1993, 262 (5140) :1718-1721

[10] TARGET ENZYME RECOGNITION BY CALMODULIN - 2.4-ANGSTROM STRUCTURE OF A CALMODULIN-PEPTIDE COMPLEX [J].

MEADOR, WE ;

MEANS, AR ;

QUIOCHO, FA .

SCIENCE, 1992, 257 (5074) :1251-1255