THE DIVA-BINDING SITE OF THE RECA PROTEIN - PHOTOCHEMICAL CROSS-LINKING OF TYR103 TO SINGLE-STRANDED-DNA

To investigate the DNA-binding site in the Escherichia coli RecA protein, RecA was covalently crosslinked to oligodeoxythymidine [p(dT)(14)] by irradiation with ultraviolet light. We identified the site of cross-linking of the protein when the RecA p(dT)(14) complex was formed in the absence of nucleotide cofactor as well as in the presence of adenosine 5'-[gamma-thio]triphosphate. When RecA p(dT)(14) complex formed without nucleotide cofactor was irradiated with ultraviolet light, a cross-linked peptide was found after digestion with Achromobactor lyticus protease I. Amino acid composition of the peptide was determined; The results indicated that the site of cross-linking was in the region spanning amino acid residues 89-106. Further digestion of the cross-linked fragment with Staphylococcus aureus V8 protease indicated that Tyr103 was the site of cross-linking. When the complex formed with adenosine 5'-[gamma-thio]triphosphate was irradiated with ultraviolet light, two cross-linked sites were detected, which were in the region of residues 89-106 and residues 178-183. These regions are far from the two disordered loops in the crystal structure, which were suggested to be DNA-binding sites by Story et al. [Story, R. M., Weber, T. W. and Steitz, T. A. (1992) Nature 355, 318-325].