RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FOR GENETIC-ANALYSIS IN MICROPROPAGATED PLANTS OF POPULUS-DELTOIDES MARSH

被引：11

作者：

RANI, V

PARIDA, A

RAINA, SN

机构：

来源：

PLANT CELL REPORTS | 1995年 / 14卷 / 07期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

RAPD markers were used to assess genetic fidelity of 23 micropropagated plants of a single clone (L34) of Populus deltoides. Eleven arbitrary 10-base primers were successfully used to amplify DNA from in vivo and in vitro material. Of these, 5 distinguished a total of 13 polymorphisms common across 6 micropropagated plants. Apart from these 6 plants, the amplification products were monomorphic across all the micropropagated plants, the mother plant and 4 additional field-grown control plants. Our results show that RAPD markers can be used to gain rapid and precise information about genetic similarities or dissimilarities in micropropagation systems that might not be so easily evident from other commonly used techniques.

引用

页码：459 / 462

页数：4

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