EFFECT OF CULTURE CONDITIONS ON GROWTH AND SPORULATION OF BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS AND DEVELOPMENT OF MEDIA FOR PRODUCTION OF THE PROTEIN CRYSTAL ENDOTOXIN

被引：39

作者：

PEARSON, D

WARD, OP

机构：

[1] DELTA BIOTECHNOL, NOTTINGHAM NG7 1FD, ENGLAND

[2] UNIV WATERLOO, DEPT BIOL, WATERLOO N2L 3G1, ONTARIO, CANADA

来源：

BIOTECHNOLOGY LETTERS | 1988年 / 10卷 / 07期

关键词：

D O I：

10.1007/BF01027055

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The influence of medium composition on the inoculum and production stages of the Bacillus thuringiensis subsp. israelensis bioinsecticide fermentation was investigated. Media which inhibited sporulation were selected for inoculum development stages. Bioinsecticide production media were designed to produce high cell counts and > 90% sporulation in a 48 h fermentation. Maximum insecticidal activity occurred at the point of maximum bacterial cell lysis/spore release. A process involving two inoculum stages and a 48 h production stage in a 40 l fermenter yielded a viable cell count of 6.5 .times. 109/ml with greater than 95% sporulation. Good correlation existed between spore counts and bioinsecticide activity.

引用

页码：451 / 456

页数：6

共 15 条

[1] PRODUCTION AND UTILIZATION OF BACILLUS-THURINGIENSIS [J].