THE CONSTRUCTION OF BACILLUS-THURINGIENSIS STRAINS EXPRESSING NOVEL ENTOMOCIDAL DELTA-ENDOTOXIN COMBINATIONS

被引:31
作者
CRICKMORE, N [1 ]
NICHOLLS, C [1 ]
EARP, DJ [1 ]
HODGMAN, TC [1 ]
ELLAR, DJ [1 ]
机构
[1] USDA ARS,CTR PLANT GENE EXPRESS,ALBANY,CA 94710
关键词
D O I
10.1042/bj2700133
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using our recently reported method of electroporation to transform Bacillus thuringiensis [Bone and Ellar (1989) FEMS Microbiol. Lett. 58, 171-178], cloned B. thuringiensis entomocidal δ-endotoxin genes have been introduced into several native B. thuringiensis strains. In many cases the resulting transformants expressed both their native toxins and the cloned toxin, producing strains with broader toxicity spectra. The introduction of the var. tenebrionis toxin gene into B. thuringiensis var. israelensis resulted in a strain with activity against Pieris brassicae (cabbage white butterfly), an activity which neither parent strain possesses. We discuss further the possibility of synergism and also the problems associated with introducing cloned DNA by this method.
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页码:133 / 136
页数:4
相关论文
共 49 条
[1]  
AHMAD W, 1990, FEMS MICROBIOL LETT, V68, P97, DOI 10.1111/j.1574-6968.1990.tb04130.x
[2]  
AHMAD W, 1989, FEMS MICROBIOL LETT, V59, P197
[3]   CLONING AND EXPRESSION OF 130-KD MOSQUITO-LARVICIDAL DELTA-ENDOTOXIN GENE OF BACILLUS-THURINGIENSIS VAR ISRAELENSIS IN ESCHERICHIA-COLI [J].
ANGSUTHANASOMBAT, C ;
CHUNGJATUPORNCHAI, W ;
KERTBUNDIT, S ;
LUXANANIL, P ;
SETTASATIAN, C ;
WILAIRAT, P ;
PANYIM, S .
MOLECULAR & GENERAL GENETICS, 1987, 208 (03) :384-389
[4]   PLASMID STRUCTURAL INSTABILITY ASSOCIATED WITH PC194 REPLICATION FUNCTIONS [J].
BALLESTER, S ;
LOPEZ, P ;
ESPINOSA, M ;
ALONSO, JC ;
LACKS, SA .
JOURNAL OF BACTERIOLOGY, 1989, 171 (05) :2271-2277
[5]   DELETION ANALYSIS OF A COMPLEX PROMOTER FOR A DEVELOPMENTALLY REGULATED GENE FROM BACILLUS-SUBTILIS [J].
BANNER, CDB ;
MORAN, CP ;
LOSICK, R .
JOURNAL OF MOLECULAR BIOLOGY, 1983, 168 (02) :351-365
[6]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[7]  
BONE EJ, 1989, FEMS MICROBIOL LETT, V58, P171, DOI 10.1111/j.1574-6968.1989.tb03039.x
[8]  
BOURGOIN C, 1988, 21ST SOC INV PATH AB
[9]   ISOLATION OF A BACILLUS-THURINGIENSIS RNA-POLYMERASE CAPABLE OF TRANSCRIBING CRYSTAL PROTEIN GENES [J].
BROWN, KL ;
WHITELEY, HR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (12) :4166-4170
[10]   ULTRASTRUCTURE, PHYSIOLOGY, AND BIOCHEMISTRY OF BACILLUS-THURINGIENSIS [J].
BULLA, LA ;
BECHTEL, DB ;
KRAMER, KJ ;
SHETHNA, YI ;
ARONSON, AI ;
FITZJAMES, PC .
CRC CRITICAL REVIEWS IN MICROBIOLOGY, 1980, 8 (02) :147-204