PREPARATION OF CHROMATOGRAPHIC SUPPORTS OF VARIABLE LIGAND DENSITY

被引：14

作者：

LANDGREBE, ME ^{[1
]}

WU, D ^{[1
]}

WALTERS, RR ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,DEPT CHEM,AMES,IA 50011

来源：

ANALYTICAL CHEMISTRY | 1986年 / 58卷 / 08期

关键词：

BIOCHEMISTRY; -; SILICA;

D O I：

10.1021/ac00121a005

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

It was possible to prepare supports containing spacer arms with terminal carboxylic acid groups, by refluxing diol-bonded silica in a solution of a cyclic anhydride. Surface densities ranging from a new percent to complete monolayer coverage were obtained by varying the reaction time, temperature, and anhydride concentration. Cation-exchange chromatography of proteins on these supports indicated that selectivity as well as retention was significantly altered by changing the surface density of exchange sites.

引用

页码：1607 / 1611

页数：5

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