THE EFFECT OF ETHANOL ON HSP70 IN CULTURED RAT GLIAL-CELLS AND IN BRAIN-AREAS OF RAT PUPS EXPOSED TO ETHANOL IN-UTERO

被引:24
作者
HOLOWNIA, A [1 ]
LEDIG, M [1 ]
COPIN, JC [1 ]
THOLEY, G [1 ]
机构
[1] CNRS,CTR NEUROCHIM,F-67084 STRASBOURG,FRANCE
关键词
FETAL ALCOHOL SYNDROME; ETHANOL; HEAT SHOCK PROTEINS; BRAIN GLIAL CELLS; RATS;
D O I
10.1007/BF00969701
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Prenatal exposure to alcohol is associated with a cluster of symptoms called Fetal Alcohol Syndrome with a characteristic pattern of neuroanatomy and biochemical changes. In recent years it has been shown, that stress exposed cells rapidly increase transcription and translation of heat shock protein genes resulting in an increased appearance of these proteins. It has also been found that heat shock proteins, especially the HSP70 family play a role as molecular chaperons maintaining the native conformation of proteins and participating in protein transport in particular cellular compartments. The aim of this study was to determine the effect of chronic maternal alcohol consumption on HSP70 content in the different regions of the brain of the newborn rats as well as to examine in vitro the effect of ethanol on HSP70 content in cultured glial cells. Chronic maternal ethanol consumption resulted in increased HSP70 in the following regions of developing brain: hippocampus, cerebellum, olfactory bulbs, frontal cortex and septum. Moreover, ethanol applied in vitro, increased HSP70 content in primary astroglial cultures and astrocytes but not in oligodendrocyte cultures. The above described changes may be important in brain maturation and may play a role in Fetal Alcohol Syndrome.
引用
收藏
页码:875 / 878
页数:4
相关论文
共 20 条
[1]  
ADICKES ED, 1988, ALCOHOL ALCOHOLISM, V33, P377
[2]  
BESNARD F, 1989, DISSECTION TISSUE CU, P136
[3]  
DRUSE MJ, 1992, ALCOHOL NEUROBIOLOGY, P1
[4]   A DUAL ROLE FOR MITOCHONDRIAL HEAT-SHOCK PROTEIN-70 IN MEMBRANE TRANSLOCATION OF PREPROTEINS [J].
GAMBILL, BD ;
VOOS, W ;
KANG, PJ ;
MIAO, BJ ;
LANGER, T ;
CRAIG, EA ;
PFANNER, N .
JOURNAL OF CELL BIOLOGY, 1993, 123 (01) :109-117
[5]   MAMMALIAN STRESS PROTEINS HSP70 AND HSP28 COINDUCED BY NICOTINE AND EITHER ETHANOL OR HEAT [J].
HAHN, GM ;
SHIU, EC ;
AUGER, EA .
MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (12) :6034-6040
[6]  
IONES DG, 1973, LANCET, V2, P989
[7]  
KIMELBERG HK, 1989, SCI AM APR, P66
[8]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[9]  
LALLEMANDERMEZG.V, 1993, BIOL PROSPECTIVE, P209
[10]   ALCOHOL AND WHITE-MATTER DEVELOPMENT - A REVIEW [J].
LANCASTER, FE .
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 1994, 18 (03) :644-647