THE SH2-CONTAINING PROTEIN-TYROSINE-PHOSPHATASE SH-PTP2 IS REQUIRED UPSTREAM OF MAP KINASE FOR EARLY XENOPUS DEVELOPMENT

被引：309

作者：

TANG, TL ^{[1
]}

FREEMAN, RM ^{[1
]}

OREILLY, AM ^{[1
]}

NEEL, BG ^{[1
]}

SOKOL, SY ^{[1
]}

机构：

[1] BETH ISRAEL HOSP, MOLEC MED UNIT, BOSTON, MA 02215 USA

来源：

CELL | 1995年 / 80卷 / 03期

关键词：

D O I：

10.1016/0092-8674(95)90498-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SH-PTP2, the vertebrate homolog of Drosophila cork screw, associates with several activated growth factor receptors, but its biological function is unknown. We assayed the effects of injection of wild-type and mutant SH-PTP2 RNAs on Xenopus embryogenesis. An internal phosphatase domain deletion (Delta P) acts as a dominant negative mutant, causing severe posterior truncations. This phenotype is rescued by SH-PTP2, but not by the closely related SH-PTP1. In ectodermal explants, Delta P blocks fibroblast growth factor (FGF)and activin-mediated induction of mesoderm and FGF-induced mitogen-activated protein (MAP) kinase activation. Our results indicate that SH-PTP2 is required for early vertebrate development, acting as a positive component in FGF signaling downstream of the FGF receptor and upstream of MAP kinase.

引用

页码：473 / 483

页数：11

共 80 条

[61] REQUIREMENTS FOR PHOSPHORYLATION OF MAP KINASE DURING MEIOSIS IN XENOPUS OOCYTES [J].